$54.81
$67.42
DescriptionThe principle of Digital PCR is to divide nucleic acids template into separate reaction chambers, allowing for individual molecule quantification through PCR amplification and fluorescence signal detection. Unlike real-time PCR, digital PCR does not rely on Ct values or standard calibration curves. It offers exceptionally high sensitivity, precision, and repeatability, making it an absolute quantitative analysis technology.The RS32 ALL IN ONE Droplet Digital PCR System puts this powerful technology at your disposal, enabling groundbreaking research at previously unattainable levels. The core principle of digital PCR is partitioning and Poisson distribution. DNA or RNA amplicons are divided into more than 20,000 droplets, with each droplet containing either 0 or 1 copy of the amplicon. After the completion of PCR thermal cycling, the droplets containing amplicons exhibit fluorescence, while those without copies do not. The number of fluorescent droplets is then counted as “0” or “1”. By employing Poisson distribution, the concentration (copy number) of the sample can be calculated based on the number of fluorescent droplets. The RS32 System utilizes advanced microfluidics technology to achieve sample partitioning, generating approximately 20,000 nanoliter-sized droplets per sample.The RS32 system is well-suited for various applications, including tumor biomarker research, copy number variation analysis, viral pathogen detection, microbial detection, genetically modified organism detection, mRNA and miRNA detection, rare nucleic acid sample detection, NGS reference standard quality control, and gene editing result verification.Product FeaturesThe industry-leading multi-fluorescence channel design enhances the functionality of the digital PCR system, facilitating multiplex target detection and acquisition of multi-channel fluorescence signals. Additionally, the system incorporates a fast and integrated automated image analysis system.ModelsSpecificationRS32044 channels , 32 samples throughputRS32055 channels , 32 samples throughputRS32066 channels , 32 samples throughputRS32077 channels , 32 samples throughputSpecificationsParameterSpecificationDroplets per Reaction20,000Droplet Volume＜1nLPCR Thermal Cycling40 cycles in＜ 90 minutes （Max heating 10℃/s , max cooling 6℃/s ）Patent technologyMono Flex™ Droplet Generation Technology, Universal Macro™ Microfluidic Flat Heating TechnologySample typeAll types of nucleic acidsSample PreparationManually load samples and droplet generation oil; also compatiblewith RainSure’s fully automatic sample preparation workstation(automated nucleic acid extraction and digital PCR cartridgeloading), no manual operation requiredSample Throughput per Run 4-32 samples/runDetection MethodCMOS, able to identify negative droplets without the need for reference dyeFluorescence Channels4 channels: FAM/SYBR Green, HEX/VIC, ROX, and CY55 channels: FAM/SYBR Green, HEX/VIC, ROX, CY5, and CY5.56 channels: FAM/SYBR Green, HEX/VIC, ROX, CY5, CY5.5, and Atto 425(or CY 7)7 channels: FAM/SYBR Green, HEX/VIC, ROX, CY5, CY5.5, Atto 425, and CY 7SensitivityDetectable mutation frequency as low as 0.001%, enabling single-copy detectionDynamic Range5 logsQuantification Precision±10%Dimension500(W)*750(D)*600(H)(mm)Weight≤ 50 kgFeaturesSupports sample recovery by applying pressure to the cartridge.Supports long-term storage and re-scanning of cartridge
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